bqt1Δ cells with persistent (>30 min) chromatin–SPB contact show bipolar spindle formation. A bqt1Δ meiocyte (h90 Atb2-GFP Sid4-GFP Hht1-mRFP bqt1Δ) was imaged by time-lapse microscopy at 27°C in an Environmental Chamber with a DeltaVision Spectris (Applied Precision) using the same settings as in Video 1.
