Myosin localization in a multiple-front 4-dpf cell after ML7 treatment. Time-lapse epifluorescence microscopy movie of the same cell as in Video 4, expressing mApple-tagged myosin light chain. Cell edge position is indicated by the colored dots, where color represents edge velocity: red is protruding, blue is retracting, according to the color bar in Fig. 6 a. Images were acquired with a wide-field microscope (Ti Eclipse; Nikon). The time stamp is in the format minutes:seconds after ML7 addition (top left). Frames were acquired every 3 s for 5 min and are displayed at 20 frames per second. Bar, 10 µm. This video corresponds to Fig. 6 b.
