GFP-K14CF–transfected K14−/− mouse keratinocytes in primary culture showing a motile behavior. K14−/− mouse keratinocytes were transfected with GFP-K14CF (green) and H2B-mCherry (red). Images were recorded using a laser scanning confocal microscope (LSM780-FCS; Carl Zeiss). Recording intervals were 5 min and the whole recording time was 6 h.