Z-stack SIM images of Cby and cilia in ciliated cells. Fully differentiated MTECs were fixed, colabeled with antibodies for Cby (green) and the ciliary axoneme marker acetylated α-tubulin (red), and imaged by superresolution 3D-SIM with a 100× objective (Nikon). A total of 33 images were collected at 125-nm z steps.
