Time-lapse video showing ablation of localized PI3K signaling by FAK inhibition in cells plated on fibronectin but not on poly-lysine, as depicted in Fig. 4 C. TIRF microscopy images of NIH 3T3 cells expressing FP-AktPH (pseudocolored), plated on either fibronectin (left) or poly-lysine (right), were acquired every 30 s for 17 min in separate experiments. At the indicated time, FAK activity was inhibited by addition of 10 µM FAK inhibitor II. Time is given in minutes and seconds. Bar, 20 µm.
