BRCA2-GFP diffusion in a live HeLa cell. Nuclei were imaged by oblique illumination using a TIRF setup (Eclipse Ti-E microscope; Nikon). In HeLa cells, BRCA2-GFP displays the same type of diffusive behavior with transient (binding) interactions and organization into particles containing more than one protein as was detected for BRCA2-GFP in ES cells. The laser beam passing into the sample had a 75° angle with respect to the objective optical axis. Frames were continuously collected for 10 s using a frame acquisition time of 50 ms. The display rate is 7 frames per second. Bar, 5 µm.
