Localization and dynamics of Tks5-GFP and F-actin on MDA-MB-231 cells seeded on labeled type I collagen fibrils. MDA-MB-231 cells constitutively expressing pLifeact-mRuby (red) and transfected with Tks5-GFP (green) were seeded on type I collagen fibrils labeled with 633-succinimidyl-ester (gray). Images were analyzed by time-lapse confocal microscopy using a laser-scanning confocal microscope (SP5 confocal microscope; Leica). Frames were taken every 4 min for 2 h.
