Live-cell invadopodia assay. WM-266-4 cells stably expressing Lifeact-GFP (green) were plated on Alexa Fluor 568–gelatin (red). Images were analyzed by time-lapse epifluorescence microscopy using an incubator microscope (VivaView FL; Olympus). Frames were taken every 30 min for 27 h. Video is played at seven frames per second.
