Disruption of the cell cortex. Time-lapse epifluorescence microscopy on a customized iMIC setup (FEI) showing disruption of the apical MV array (left, cells stably transfected with Lifeact-GFP) and myosin network (right, cells stably transfected with MHCA-GFP) at the apical surface of MDCK cells, upon treatment with 2 µM latrunculin A. Maximum projections of three (for actin, Δz = 1.5 µm) or two (for myosin, Δz = 1 µm) focal planes are shown. Bar, 2 µm. Frames were taken every 20 s for 15 min. Video to Fig. 7 (A and B).
