LTBP-1 organization by myofibroblasts in co-culture with HEK293 cells. hDMfs were transfected with pLifeAct-RFP (gift from B. Geiger, Weizmann Institute, Rehovot, Israel) and seeded on top of 7-d-old cultures of LTBP-1–EGFP-expressing HEK293. hDMfs were allowed to adhere for 4 h before video recording was performed for 1 h with images taken every 60 s. GFP was detected at 488 nm (green), LifeAct-RFP was detected at 568 nm (red), and the cell layer was visualized in phase contrast. Images were taken on an upright microscope (Axio Imager) equipped with a camera (AxioCam HRm; Carl Zeiss).
