GFP-Arp1A localizes to new minus ends and moves with them to poles. Time-lapse spinning disk confocal movie of a metaphase PtK2 cell transfected with mCherry-tubulin (red) and GFP-Arp1A (green). Arp1A localizes to new minus ends and moves processively poleward with them after laser ablation (X; time 00:00). Arrowhead highlights the movement of Arp1A puncta after ablation. The kinetochore of the ablated k-fiber is marked by an asterisk. Time in min:s. Video was collected using a spinning disk confocal microscope (CSU-X1 with Eclipse Ti-E) at a rate of 1 frame every 10 or 5 s and is played back at a rate of 30× real time (see Materials and methods). Video corresponds to the still images from Fig. 5 C.
