Aurora A tracks with MCAK-labeled MT plus ends. Live-cell imaging of Em–Aurora A (left) and mCherry-MCAK (middle) and overlay (right) reveal that Em–Aurora A (green) tracks with a proportion of mCherry-MCAK (red) on growing MT plus ends at the leading edge of wound-edge HUVECs. In addition to growing MCAK-labeled MT plus ends, Em–Aurora A labeling is also visible on filaments behind the growing MCAK-labeled MT plus ends. Images were acquired by time-lapse spinning disk confocal microscopy (Yokogawa CSU-X1 spinning disk Andor Technology; coupled to a TiE microscope Nikon). Frames were taken at 2-s intervals for 2 min using a 500-ms exposure for the 488-nm (green) and a 600-ms exposure for the 560-nm (red) excitation. Bar, 2 µm.
