Tracking of MCF10A overexpressing or not overexpressing PDK1 during wound enclosure. MCF10A cells were infected with lentivirus coding for PDK1_WT or empty vector and tested with a wound healing assay. Time-lapse images were taken via fluorescence microscopy (LAS AF 6500/7000, 10× objective lens, equipped with a humidified chamber at 37°C and 5% CO2; Leica) with a frame rate of 20 min per frame for a total time of 11 h. Cells were tracked with MetaMorph 7.0 software.
