Tracking of MCF10A silenced or unsilenced for PDK1 during wound enclosure. MCF10A cells were infected with lentivirus coding for shScr, shPDK1 #79, or shPDK1 #81 and contemporary with a GFP-expressing lentivirus, then tested with a wound healing assay. Time-lapse images were taken by fluorescence microscopy (LAS AF 6500/7000, 10× objective lens, equipped with a humidified chamber at 37°C and 5% CO2; Leica) with an frame rate of 20 min per frame for a total time of 11 h. Cells were tracked with MetaMorph 7.0 software (Molecular Devices).
