Midzone microtubule dynamics of a full-length anaphase spindle in a control cell. The video shows fluorescence recovery in a budding yeast spindle midzone after photobleaching GFP-tubulin in a cdc15-2 cell maintained at 37°C. Pre-bleach and post-bleach frames were extended to allow clear observation. Arrow indicates location of bleached zone. Images were acquired at 5-s intervals using an EM-CCD camera (Cascade 512B; Photometrics) and a spinning-disk confocal attachment (CSU10; Yokogawa Corporation of America) mounted on a microscope (Axiovert 200M; Carl Zeiss). The microscope and photobleaching laser (MicroPoint; Photonics Instruments, Inc.) were controlled by MetaMorph software (Molecular Devices). Playback speed is 15 frames/s.
