Electron tomogram of stimulated shi12-12B/Y; shi-4C/+ larvae at restrictive temperature after FALI. Video shows an electron tomogram of a stimulated bouton of shi12-12B/Y; shi-4C/+ larvae at restrictive temperature after FALI. A tilt series of a 300-nm-thick section was collected from −52 to 60° with 2° angular increments in a transmission electron microscope (JEM-2100; JEOL) at 10,000× using Recorder software (JEOL) and a 1,024 × 1,024–pixel bottom-mounted charge-coupled device camera (MultiScan; Gatan). Tomograms were reconstructed with the eTomo module in IMOD. Each video frame in the tomogram is an individual slice (54 slices in total). The surface-rendered model, built by semiautomated surface rendering and computed using 3dmod in IMOD, is shown in Fig. 7. Note the accumulation of membrane inclusions that are also connected to the presynaptic membrane. Bar, 200 nm.
