Fluorescence recovery after photobleaching of a wortmannin-injected embryo expressing YFP::Bnk. FRAP of a wortmannin injected embryo during the slow phase of cellularization imaged by two-photon microscopy (LSM 780 NLO; Carl Zeiss). White box indicates the bleached region; YFP::Bnk (white). Frames were acquired every 400 ms for 2 min. Bar, 10 µm