Localization of Hok1-GFP on mCherry-Rab5a–labeled EEs in hyphal cells of U. maydis. Hok1-GFP (green) is always present on the bidirectionally moving organelles (red). Cells were placed on an agar cushion, and image series were taken immediately using a charge-coupled device camera (CoolSNAP HQ2; Photometrics) and a laser-based inverted microscope (IX81 [Olympus] equipped with a VS-LMS4 Laser Merge System [Visitron Systems] and controlled by the software MetaMorph). Frames were taken every 150 ms for 14.9 s. Images were adjusted in brightness, contrast, and γ settings. Time in seconds and milliseconds; bar is in micrometers.
