Diffusing type 2 nodes in five cells at progressive stages of interphase. Reverse contrast sum intensity projections of three consecutive confocal z sections of five fission yeast cells expressing Blt1p-mEGFP in progressive stages of interphase with elapsed time expressed as minutes and seconds. Stage of interphase estimated from cell length (see Materials methods). The leftmost image is of two daughter cells still connected by a septum. Note the accumulation of stationary, equatorial nodes as cells advanced through interphase, and the concomitant decrease of fast-moving nodes, particularly at the new cell tip. Frames were taken every 2 s for 200 s. Oval lines outline cells. Images were acquired with a spinning-disk (CSU-X1; Yokogawa Corporation of America) confocal microscope (IX-71; Olympus) and an electron-multiplying charge-coupled device camera (iXon 897; Andor Technology).
