PIP3 and F-actin dynamics during re-orientation of a cell to a micropipette releasing cyclic-AMP. An aggregation-competent Ax2 cell expressing PH-CRAC-GFP (green) as a reporter for PIP3, and LifeAct-RFP (red) as a reporter for F-actin, is induced to change direction by moving a micropipette releasing cyclic-AMP. A PIP3 patch and F-actin microspikes are produced proximal to the needle and are followed in this case by a bleb formed from the PIP3 patch. DIC and fluorescent images were taken by laser-scanning confocal microscopy (model LSM 780; Carl Zeiss) at 1 frame per second.
