Blebbing assay. In this assay, 1 µM cyclic-AMP is added to aggregation-competent Ax2 cells, expressing ABD-GFP as a reporter for F-actin. Cyclic-AMP addition is marked by a disturbance to the image (0 s), and blebbing starts 20 s later. DIC and fluorescent images were taken by laser-scanning confocal microscopy (model LSM 710; Carl Zeiss) at 1 frame per second.
