ProActive analysis of a D16C3 control cell. Cell body movements after ProActive analysis of the D16C3 cell transfected with GFP-actin that is shown in Video 3. Images were acquired by time-lapse confocal microscopy using a laser spinning-disc microscope (Wallac UltraVIEW; PerkinElmer) and 100×, 1.4 NA oil objective (Nikon). Frames were taken every 2 s for 3.5 min. Filopodia are removed, and cell body outlines are overlaid to reveal cell body dynamics at a lag of 3, i.e., frame 1 is time t (red) and time t + 3 (blue), frame 2 is t + 1 (red) and t + 4 (blue), etc. Areas of protrusion are in white, and areas of retraction are in black.
