FiloTrack analysis of a Drosophila D16C3 control cell. D16C3 cell transfected with GFP-actin and overlaid with the cell boundary (blue) and filopodia (red) after FiloTrack analysis. When protrusions are below the set critical filopodia length, FiloTrack marks them in yellow. Images were acquired by time-lapse confocal microscopy using a laser spinning-disc microscope (Wallac UltraVIEW; PerkinElmer) and 100×, 1.4 NA oil objective (Nikon). Frames were taken every 2 s for 3.5 min.
