Drosophila D16C3 cell with a large number of filopodia identified using CellGeo. A Drosophila D16C3 cell cotransfected with mCh-actin (white) and GFP-Dia ΔDAD (not depicted) overlaid with the cell boundary (blue) and filopodia (red) after FiloTrack analysis. Protrusions are tracked and marked as longer (red) or shorter (yellow) than the critical filopodia length. Images were acquired by time-lapse confocal microscopy using a laser spinning-disc microscope (Wallac UltraVIEW; PerkinElmer) and 100×, 1.4 NA oil objective (Nikon). Frames were taken every 2 s for 7 min.
