Kinetochore oscillations in control HeLa cells. Time-lapse sequence of a control HeLa cell treated with luciferase siRNA expressing EGFP-CENP-B and EGFP-centrin. Images were collected every 3 s for 10 min on an imaging system (DeltaVision Personal DV; Applied Precision). The movie is a projection of five optical sections played back at 20 frames per second (Related to Fig. 2).