Confocal time-lapse video showing HeLa cell collapse. HeLa cells were transfected with wtEphB2-3FKBP-YFP and myr-mCherry (for better visualization) and stimulated with AP20187 (250 nM) immediately before the start of confocal time-lapse acquisition. Images were recorded by time-lapse confocal microscopy (Fluoview FV1000; Olympus) equipped with an Argon ion laser. Acquisition at 1 frame/min. Fluorescence intensity is shown. Bar, 10 µm. The HeLa cell shows a characteristic response pattern with contraction and respreading upon clustering with AP20187. Approximately 30 min after stimulation, internalized EphB2-loaded vesicles become visible (see also Fig. S4).
