Example of metaphase HeLa cells expressing Zwint1-GFP/Hec1-tdTomato used to make live Delta measurements. Double thymidine-synchronized HeLa cells were transfected with cDNA plasmids encoding Zwint1-GFP and Hec1-tdTomato and subjected to continuous tandem two-color live imaging during metaphase using an inverted microscope (TE300). Videos were generated from merged z stacks of images obtained from both the channels where each frame was played for 1/6 s.
