FA maturation in control and Vcl-KO MEF. SDC fluorescence time-lapse image sequences of maturing FA in control and Vcl-KO MEF expressing EGFP-paxillin. Arrows indicate FA shown in Fig. 5 H. Images were taken every 20 s (elapsed time shown in min:s) on an inverted microscope system (TE2000E2; Nikon) using a 100×/1.49 NA Apo TIRF objective lens. Bar, 2 µm.