Overlay of F-actin flow and FA in control and Vcl-KO MEF. SDC fluorescence time-lapse image sequences of FA (EGFP-paxillin; left), overlayed with F-actin flow maps (right) in control and Vcl-KO MEF. Images were taken every 10 s (elapsed time shown in min:s) on an inverted microscope system (TE2000E2; Nikon) using a 100×/1.49 NA Apo TIRF objective lens. Bars: 2 µm; (flow scale) 2 µm*min−1.
