Rac activity is high at the cell front under basal conditions as cells migrate on CDM. A2780 cells were transfected with Raichu-Rac and seeded onto CDMs after 16–24 h, and fluorescence lifetime images were captured at 1-min intervals using the Marianas inverted microscope system and a 63× objective. Videos of lifetime maps are presented, with colder colors representing low lifetime (and high activity) and warm colors representing high lifetime (and low activity).
