Deleting snph abolishes activity-dependent immobilization of axonal mitochondria. Representative time-lapse images showing the relative motility of both mitochondria (red) and late endosomes (green) along the same axons of snph−/− neurons before and after brief treatment with 50 mM KCl and 10 µM FPL 64176. Hippocampal snph−/− neurons were cotransfected with DsRed-Mito and GFP-Rab7 at DIV9 followed by ∼16-min time-lapse imaging at DIV12 (8 min each before and after treatment). Time-lapse sequences of 100 frames were collected at 10-s intervals using a laser-scanning confocal microscope (LSM 510 META). Movement toward the bottom indicates retrograde transport. The kymograph of this video is presented in Fig. 1 B.
