Composite of representative time-lapse movies of apical anchorage of Alexa Fluor 488–labeled h RNA in WT and clip190KO/Df-null mutant embryos (apical views). WT, left; clip190 mutant, right. Image series of cross sections of the apical cytoplasm, beginning ∼4 min after RNA injection. In both genotypes h RNA puncta (white) are retained in the apical cytoplasm. Video has a duration of 37.5 s and a height corresponding to 15.5 µm. One frame was captured every 297 ms with a spinning disk microscope (Ultraview ERS; PerkinElmer) equipped with a 60×/1.2 NA UPlanApo water objective. Images were captured at ∼22°C.
