Emigration of HT1080/MT1 cells from multicellular spheroids into bovine (left) or rat (right) collagen (1.7 mg/ml) in the absence (A, first sequence) or presence (B, second sequence) of MMP inhibitor GM6001 with paths representing the position change of cell centers. Representative still images are shown in Fig. S3 A. Bright-field inverse microscopy (Leica) at 37°C; time (h:min:s) as indicated (16-min intervals over 8.5 h of observation).
