msn-RNAi mosaic egg chambers exhibit a cell-autonomous defect in follicle cell migration. The cells in an msn-RNAi flipout clone (GFP) move very little while neighboring wild-type cells efficiently migrate past them (Fig. 1 F). The mosaic egg chamber was analyzed by time-lapse microscopy using a spinning disk confocal microscope (Axiovert 200M; Carl Zeiss) with a spinning disk unit (model CSU-10; Yokogawa Corporation of America). Cell membranes are labeled with FM4-64 (red). Frames were taken every 30 s for 32 min. Playback rate is 300× real time.
