A large msn mutant clone blocks global follicle cell migration. msn172 cells (yellow dots) are stationary, while neighboring wild-type cells attempt to move past them (Fig. 1 D). The msn mosaic egg chamber was analyzed by time-lapse microscopy using a spinning disk confocal microscope (Axiovert 200M; Carl Zeiss) with a spinning disk unit (model CSU-10; Yokogawa Corporation of America). Cell membranes are labeled with FM4-64. Frames were taken every 30 s for 61 min. Playback rate is 300× real time.
