CD147 and Hook1 colocalize on tubular endosomes and endosomal structures. After antibody internalization (30 min at 37°C), endogenous CD147 (green; Alexa Fluor 488) and endogenous Hook1 (red; Alexa Fluor 568) were visualized using super-resolution fluorescence microscopy in HeLa cells (SIM; DeltaVision OMX V4; Applied Precision). The video shows a 3D SIM image of internalized CD147 (green) with Hook1 (red) rotated from 0° to 14° (from Fig. 4 C).
