Dynamics of microtubules and telomeres in cells undergoing cell conjugation. Cells of opposite mating types expressing mCherry-tagged Taz1 (magenta) and GFP-tagged Atb2 (green) were induced to enter meiosis on solid ME medium. Time-lapse images were collected using a fluorescence microscope (IX71) equipped with a 60×/1.42 NA Plan Apochromat oil immersion objective lens and a cooled CCD camera (CoolSNAP-HQ2). Images at seven focal planes spaced at 0.5-µm intervals were taken every 30 s for 30 min, and each set of images was processed by deconvolution and combined to form a maximal projection by MetaMorph software. The video plays at a rate of three frames per second. The video sequence shows clustering of telomeres with microtubules. The two cells eventually fuse, and mCherry-tagged Taz1 diffused in the nucleus in one of the conjugating cells (top right) because it inefficiently localized to telomeres as a result of the expression of endogenous Taz1.
