FRAP sequence with DEE mutant VE-cadherin. False-color time-lapse video of RFP-tagged DEE mutant VE-cadherin expressed in primary human dermal microvascular endothelial cells. The video begins shortly before bleaching of a 5-µm-long section of the junction (arrow) and continues for 10 min after the bleach. Images were captured by laser-scanning confocal microscopy (A1R) every 15 s and are 31.9 µm wide. Individual frames are shown in Fig. 8 A. VE-cad, VE-cadherin.