Coordinated F-actin and myosin dynamics during cell periodic protrusions. Time-lapse imaging data demonstrates cytoskeletal dynamics during oscillation. Merged fluorescent image of F-actin (Lifeact-GFP, green) and myosin (MyosinRLC-RFP, red). Note the striking repeatability of the F-actin and myosin distributions in each period. Video was recorded on a Nipkow-type spinning disk confocal scan head with a 60×, 1.40 NA objective and a 1,024 × 1,024 camera. Images were aquired at 1-s intervals and play back at 10 fps.
