Myosin VI NI isoform lacks the ability to cage SG movement at the plasma membrane. Myosin VI stable knockdown PC12 cells cotransfected with GFP-MyoVI-SIfull and NPY-mCherry were visualized after nicotine stimulation using time-lapse live-cell imaging using a TIRF microscope (Marianas and Everest; Intelligent Imaging Innovations, Inc.). The trajectory of a NPY-mCherry–positive SG is not restricted upon colocalization with GFP-MyoVI-NIfull. The video was compiled at two frames per second.
