HeLa Kyoto cell stably expressing GFP–α-tubulin as well as mCherry-H2B HeLa Kyoto cells treated with pertussis toxin or transfected with GFP-NuMA(1–705)-CAAX plus treated with pertussis toxin. S21 shows a pertussis toxin–treated cell; S22 shows GFP-NuMA(1–705)-CAAX plus pertussis toxin. Time-lapse videos were acquired using a laser-scanning confocal microscope (LSM 700) at one frame every 3 min and played at 3 frames/s. Note that treatment with pertussis toxin (PT) does not impair spindle oscillations upon GFP-NuMA(1–705)-CAAX expression (S22).
