Time-lapse DIC microscopy of wild-type, YFP–GPR-1, or YFP–GPR-1 dhc-1(RNAi) one-cell stage C. elegans embryos. S12 shows wild type; S13 shows YFP–GPR-1; S14 shows YFP–GPR-1 dhc-1(RNAi).Anterior is on the left, and embryos are ∼50 µM long. Time-lapse videos were acquired using differential interference contrast microscopy (Axioskop 2 Plus; Carl Zeiss) at one frame every 5 s and played at 10 frames/s. Note excess spindle oscillations in S13 only.
