Video 3.

HeLa Kyoto cell stably expressing GFP–α-tubulin as well as mCherry-H2B and transfected with Gαi₁-YFP plus p150^Glued siRNAs, YFP-LGN plus p150^Glued siRNAs, Gαi₁-YFP plus DYNC1H1 siRNAs, or YFP-LGN and DYNC1H1 siRNAs. S7 shows Gαi₁-YFP plus p150^Glued siRNAs; S8 shows YFP-LGN plus p150^Glued siRNAs; S9 shows Gαi₁-YFP plus DYNC1H1 siRNAs; S10 shows YFP-LGN and DYNC1H1 siRNAs. Time-lapse videos were acquired using a laser-scanning confocal microscope (LSM 700) at one frame every 3 min and played at 3 frames/s.

Cortical dynein is critical for proper spindle positioning in human cells

Sachin Kotak, Coralie Busso, and Pierre Gönczy

DOI: 10.1083/jcb.201203166