HeLa Kyoto cell stably expressing GFP–α-tubulin as well as mCherry-H2B and transfected with YFP or YFP-LGN. S5 shows YFP; S6 shows YFP-LGN. Time-lapse videos were acquired using a laser-scanning confocal microscope (LSM 700) at one frame every 6 s and played at 3 frames/s.