Chromosome alignment and segregation defects upon overexpression of GFP-CLASP2 S1234A mutant. (Related to Fig. 6 B) HeLa cell–overexpressing GFP-CLASP2 S1234A mutant (green and video in the middle) with chromosomes marked with mCherry-H2B Histone (red and video on the right), followed by spinning disk confocal microscopy (Andor based on an Olympus IX81). Each frame represents a maximal intensity projection acquired every 3 min. Overexpression of GFP-CLASP2 S1234A mutant causes a significant delay in mitosis and cells eventually enter anaphase in the presence of misaligned chromosomes. During anaphase, chromatin bridges are also visible, followed by cytokinesis failure. Time is shown in hours:minutes.
