Phosphorylation of CLASP2 on S1234 by Cdk1 is required to maintain spindle bipolarity. (Related to Fig. 5, C and C’) HeLa mCherry-H2B Histone (red) stable cells were transfected with GFP-CLASP2 S1234A mutant (green), and mitotic progression was followed by spinning disk confocal microscopy (Andor based on an Olympus IX81). Video illustrates how S1234A mutant overexpression induces mitotic spindle collapse. GFP-CLASP2 S1234A signal alone is shown on the right after contrast inversion; each frame represents a maximal intensity projection acquired every 3 min. Time is shown in hours:minutes.
