mCherry-MT1-MMP vesicles polarized trafficking to invasive pseudopods. MDA-MB-231 cells treated with siRNA of NT (left) and N-WASP #1 (right) were cotransfected with mCherry-MT1-MMP (red) and GFP-Lifeact (green). The cells then were overlaid with Matrigel and imaged with a laser-scanning confocal microscope (Fluoview FV1000; Olympus) a uPlan-SApochromat 60×/1.35 NA oil objective lens. The movie shows that mCherry-MT1-MMP vesicles polarized traffic to invading pseudopods in NT cells while invading in CIA, but not in N-WASP knockdown cells. The arrow indicates the wound direction in CIA.
