Tracking of endodermal cell migration during gastrulation. The lateral marginal region of a Tg(sox17:GFP-UTRN) embryo was imaged from 6–9 h after fertilization. Dorsal is to the right. Images were analyzed by time-lapse confocal microscopy using a microscope (Ti-E) equipped with a spinning-disk confocal unit (CSU-22). Frames were acquired every minute for 2.5 h. To illustrate the switch from random to oriented migration, one cell was tracked for the duration of the video using the Manual Tracking plugin in ImageJ. Playback is 10 frames/s.
