Spindle formation and positioning in DYNLL1-depleted cells. HeLa cells stably expressing GFP-tubulin and mCherry-histone H2B were transfected with DYNLL1 siRNA duplexes for 72 h, and were then used for time-lapse imaging. Images corresponding to 29 planes spaced by 0.6 µm through the cell volume were collected every minute as the cells passed through mitosis using a spinning-disk confocal microscope (UltraView Vox; PerkinElmer). Maximum intensity projections of tubulin (green) and histone H2B (red) are shown through time; each frame corresponds to 1 min.
