Morphology of K-Ras V12 Caco-2 3D structure. A Caco-2 3D culture expressing K-Ras V12 (pQCXIP GFP K-Ras V12) was fixed at day 10 and stained for actin (red) and DNA (blue). 200 images comprising a z stack spanning 106 µm were acquired with a spinning-disk confocal microscope (UltraVIEW ERS) and used to make a stack video with MetaMorph.
